| 1. | Flow and heat transfer of mixture of solid media and biomass in inclined tube
固体热载体和生物质粉沿倾斜管流动和传热的计算 |
| 2. | Microbiological analysis of milk ; determination of coliforms ; method with solid medium
牛奶的微生物检验.用固定培养基测定大肠杆菌状细菌 |
| 3. | Input seismic wave fields in elastic half - space interbedded by liquid - filled porous medium and solid medium
不同介质互层分布的弹性半空间地震波场输入 |
| 4. | Positive transformants were also detected by kan resistant re - screening on solid medium or directly detection in field and southern blot
利用卡那霉素抗性再筛选及kan抗性大田直接筛选、 southernblot等方法对阳性植株作了进一步检测。 |
| 5. | Either instrument can be used to obtain the inoculum from an agar slant culture by carefully touching the surface of the solid medium in an area exhibiting growth so as not to gouge into the agar
任何一种器具都可以用来在洋菜斜面培养上获得接种原，小心地碰触有生长的固体培养基的表面，避免刺破洋菜培养基。 |
| 6. | This system included method of solid medium plate enveloped with parafilm to form colony , conjugational gene transfer in a selective medium , screening the nonmagnetic mutants by magnet adsorption technique
此体系包括：以平板封膜培养技术获得单菌落，在选择性培养液中进行遗传因子接合转移，以液体培养和磁铁吸附技术筛选突变子。 |
| 7. | Sporocytophaga is a kind of bacteria that can glide on the surface of solid medium . this strain can decompose the cellulose strongly . lt can grow on the surface of cotton and fiter paper , and produce large amount of extracellular polysacchrides during the cellulose degradation . the sporocytophag can only produce a low extracellular carboxymethyl - cellulase ( cmc ) activity and no other extracellular cellulase activities . so the sporocytophaga has a special mechanism of cellulose degradation
生孢噬纤维细菌通过与纤维素物质的紧密粘附作用而强烈地降解纤维素；但该菌只能测到极低的胞外cmc酶活，而没有其他的胞外纤维素酶活，因此该菌有特殊的纤维素降解机制。 |
| 8. | The results showed : the best additive water to wheat bran solid medium was 35 percent . a . niger an01001 produced high activity phytase when it was cultivated at 30 ? from 90 to 120 hours , and obtained the highest enzyme activity at 114 hours . phytase activity was effected by different buffer and ph , enzyme activity was high at ph 5 . 5 and 6
对该菌的产酶条件进行研究的结果表明：麸皮固体培养基的最适加水量为35 ； 30培养90 120h之间产酶都比较高，在114h酶活最高；在提取时不仅ph对酶得率有影响，而且不同提取液所要求的最适ph也不同，用乙酸缓冲液提取时，当ph为5 . 5 、 6 . 5时酶活较高，进行水提取时，在ph7 . 5时酶活最高；利用cacl _ 2溶液进行提取的浓度达到3时，酶活最高。 |
| 9. | Purification and characterization of phytase from a . niger an 01001 a . niger an01001 was inoculated on solid media and cultivated at 30 for 5 days . proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5 . 5 ) . the molecule weight of the phytase protein was determined as about 78kd by sds - page . the purification procedures include ammonium sulfate precipitation , deae - cellulose ion - exchange chromatography , gel electrophoresis and electroelution
3 .植酸酶的分离纯化及其性质研究黑曲霉ano1001经固体发酵，用缓冲液抽提后，经硫酸按沉淀， deae一纤维素离子交换层析，聚丙烯酞胺凝胶电泳和电洗脱等纯化步骤获得的植酸酶，用sds一page检测为一条均一谱带，其分子量约为78kd 。 |
| 10. | Thirdly , the hyl gene was cloned to puc19 and pbr322 respectively . then the fragment containing ampr derived from puc19 and hasa came from pse380 - has were inserted into hyl either or both in vitro . transformed the recombinated vectors into s . equi by electro - operation , then plating on hag solid medium containing ampicillin , and selected clones hi which the hyl gene was inactivated by gene replacement through homologous recombination
( 1 )直接插入外源基因灭活hyl方法：将hyl片段与载体puc19 pbr322连接后，用来自puc19的amp ~ r抗性基因片段从hyl基因的中部插入将载体上的hyl片段分为两部分； ( 2 )用hasa替换hyl部分片段的方法：将hyl片段与载体pucl9 pbr322连接后，用hasa片段替换部分hyl ，再将amp ~ r片段接入到hasa上游。 |
